Cell-ACDC output data

Files saved by Cell-ACDC for a fully analysed experiment (example with original raw microscopy file called Example1 and first position _s01_)

File:

Description:

Example1_s01_acdc_output.csv

Main table containing cell cycle annotations and additional metrics such as mean, median etc. for all the loaded channels plus all the region properties (as calculated by skimage.measure.regionprops) for each segmented object.

Example1_s01_act1.tif

.tif file for the channel called act1.

Example1_s01_cdc10.tif

.tif file for the channel called cdc10.

Example1_s01_last_tracked_i.txt

Last visited frame in “Segmentation and Tracking mode” with the main GUI.

Example1_s01_metadata.csv

Table containing the metadata such as number of frames, number of z-slices, pixel size etc.

Example1_s01_phase_contr.tif

.tif file for the channel called phase_contr.

Example1_s01_segm.npz

Segmentation labels. This is a numpy array (compressed).

Example1_s01_segmInfo.csv

Table containing information such as which z-slice or z-projection was used for segmentation or saving metrics of each channel.

Example1_s01_align_shift.npy

Numpy array containing the shifts applied to each frame when aligning. Useful for reverting to non-aligned state.

Example1_s01_dataPrepROIs_coords.csv

Table containing the coordinates of the ROI that was used to either crop, or segment only objects in the ROI. This is created during the data prep or segmentation stage.

Example1_s01_phase_contr_aligned.npz

Aligned data for the channel called phase_contr. This is a numpy array (compressed).

Example1_s01_phase_contr
_aligned_bkgrRoiData.npz

Data from the background ROIs generated at the data prep stage.

Example1_s01_phase_contr
_dataPrep_bkgrROIs.json

Coordinates of the background ROIs generated at the data prep stage.